Pachnolepia pruinata is a rare species that is known from two localities in Agder and Vestland. It is characterized by immersed angular, rounded or lobed apothecia with thick white pruina, and a white to pale olive grey, C+ red thallus with trentepohlioid photobiont. The colorless spores are (3–)4–5 transversely septate. The apical cell is not enlarged.

Pachnolepia pruinata collected from Quercus sp. in Blekinge, Sweden in 1872 (O-L-204389).

Description

Thallus

The extensive thallus is white to pale olive grey, cracked to fissured-areolate, and has a matt to powdery surface. It is up to 0.3 mm thick and developed mainly on the surface of the bark. The margin is not determinate or delimited by a thin brown line when in contact with other lichens. The photobiont is a species of the family Trentepohliaceae.

Fruitbodies

The apothecia are usually level with the thallus surface but can be raised. They are rounded, angular or star-shaped, and colored pale brown to brown beneath a thick white pruina. They are 0.2–1 mm in size and 100–160(–250) μm tall. The disc is flat to slightly convex.

The epithecium is 10–20 μm tall, reddish brown in color, and densely interspersed with or overlain by calcium oxalate crystals.

The hymenium is colorless and 45–60 μm tall.

The hypothecium and the hymenium can be unpigmented or a pale reddish brown, and 50–170 μm tall.

The paraphysoids are 1–1.5 μm wide. Their tips are mainly perpendicular to the surface, partly free, and slightly widened to 2.5 µm. Sparse reddish brown pigment is usually deposited on the outer walls.

The asci are clavate, with stipe, 32–50 × 10–20 μm in size, and 8-spored.

The spores are persistently colorless, narrow obovate, are 13–17(–20) × 4.5–7 μm in size and divided by (3–)4–5 transverse septa. The apical cell is not enlarged.

Anamorph

The pycnidia are pale to reddish brown, 0.1–0.2 mm in size, and either immersed in the thallus or slightly raised. The wall is either colorless or a pale reddish brown. The conidia are 10–14 × 0.5–0.8 μm in size, short threadlike, and usually curved.

Chemistry

The thallus in most specimens reacts C+ red, K–, KC–, Pd–, or UV–. Arthoniaic acid is detected by TLC.

The hymenium reacts I+ blue then red and KI+ deep blue. A KI+ blue ring structure is present in the tholus of the asci. Calcium oxalate crystals are common in thallus and apothecia.

The brown pigment in the epithecium and in the wall of the pycnidia changes to pale green in K solution.

Ecology

Pachnolepia pruinata is a warm-temperate species that preferably grows on the dry, rain-shaded bark of old trees in open agricultural landscape, woodlands, and well-illuminated forests. The species is most common on old oak (Quercus spp.) trees but can be found on other substrates as well including acorn (Acer spp.), European ash (Fraxinus excelsior), worked wood and even dry stonework. Pachnolepia pruinata has become increasingly rare in parts of central Europe.

In Norway, P. pruinata has been found only once in an oak dominated deciduous forests. It grew on the trunk of an oak tree shaded from direct rain by an overarching rock wall.

Distribution in Norway and the Nordic countries

Pachnolepia pruinata is in Norway only known from two distant localities in Kvinesdal municipality (Agder) and Gulen municipality (Vestland). In the Nordic countries, it is further known from Denmark and southern Sweden.

Global distribution

Pachnolepia pruinata is widespread in the warm-temperate regions of Europe, northern Africa and North America.  

Similar species

Pachnolepia pruinata is a distinctive species that cannot easily be confused with any other lichen in Norway.

Remarks

The only collection of P. pruinata in Norway lacks arthoniaic acid and the thallus reacts C–. The morphological characters of this collection leave no doubt about its identification. Pachnolepia pruinata should be searched for in suitable habitats including Quercus dominated forests, woodlands, and solitary trees in open agricultural landscape in southern and western Norway.

Literature

Frisch A, Thor G, Ertz D and Grube M (2014). The Arthonialean challenge: restructuring Arthoniaceae. Taxon 63: 727–744.

Frisch A, Klepsland J, Palice Z, Bendiksby M, Tønsberg T and Holien H (2020). New and noteworthy lichens and lichenicolous fungi from Norway. Graphis Scripta 32(1): 1–47.